Plasma hydroxyproline-containing protein (hypro-protein)

Clinical Significance of a Hydroxyproline-containing Protein in Human Plasma.

Recently, we have identified a hydroxyprolinecontaining protein(s) in human plasma (1) and developed a specific method for its assay (2). The behavior of this protein (hyproprotein) as shown in precipitation and gel filtration experiments indicates that it is a large molecule; its resistance to digestion by proteolytic enzymes suggests further that it may be a form of collagen (2). As part of a...

A Hydroxyproline-containing, Collagen-like Protein in Plasma and a Procedure for Its Assay.

The presence of dialyzable hydroxyproline-containing peptides in plasma (1) and urine (2-4) has been known for some t,ime. Although their exact significance is not clear, the levels of these peptides in the absence of collagen or gelatin in the diet are a reflection of endogenous collagen metabolism (l-5). In a previous publication (6) it was shown that, in addition to free hydroxyproline and d...

Separation of hydroxyproline-containing protein from C1q (a subcomponent of complement) in serum.

Precipitation of the euglobulin fraction from serum separates C1q from another hydroxyproline-containing protein(s), which remains in the serum-minus-euglobulin fraction. The presence of C1q in the euglobulin fraction has been verified by radial immunodiffusion assay, by sensitivity to collagenase, by hydroxyproline content, and by electrofocusing. The presence of another hydroxyproline-contain...

Access and Rational use of Plasma Protein Therapies (PPTs)

Characterization of mercury-containing protein in human plasma.

Characterization of mercury binding protein in the human body is very important for understanding the metabolism and the mechanism of toxication of ingested mercuric compounds. In this study, mercury-containing protein in human plasma was separated by on-line heart-cutting two-dimensional high performance liquid chromatography (2D-HPLC). This 2D separation system used size exclusion liquid chro...